Characterization of erythrose reductase from Yarrowia lipolytica and its influence on erythritol synthesis.

ABSTRACT:

Background

Erythritol is a natural sweetener that is used in the food industry. It is produced as an osmoprotectant by bacteria and yeast. Due to its chemical properties, it does not change the insulin level in the blood, and therefore it can be safely used by diabetics. Previously, it has been shown that erythrose reductase (ER), which catalyzes the final step, plays a crucial role in erythritol synthesis. ER reduces erythrose to erythritol with NAD(P)H as a cofactor. Despite many studies on erythritol synthesis by Yarrowia lipolytica, the enzymes involved in this metabolic pathway have ever been described.

Results

The gene YALI0F18590g encoding the predicted erythrose reductase from Y. lipolytica was overexpressed, and its influence on erythritol synthesis was studied. The

SUBMITTER: Janek T 

PROVIDER: S-EPMC5504726 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature