PAT-H-MS coupled with laser microdissection to study histone post-translational modifications in selected cell populations from pathology samples.

ABSTRACT:

Background

Aberrations in histone post-translational modifications (hPTMs) have been linked with various pathologies, including cancer, and could not only represent useful biomarkers but also suggest possible targetable epigenetic mechanisms. We have recently developed an approach, termed pathology tissue analysis of histones by mass spectrometry (PAT-H-MS), that allows performing a comprehensive and quantitative analysis of histone PTMs from formalin-fixed paraffin-embedded pathology samples. Despite its great potential, the application of this technique is limited by tissue heterogeneity.

Methods

In this study, we further implemented the PAT-H-MS approach by coupling it with techniques aimed at reducing sample heterogeneity and selecting specific portions or cell populatio

SUBMITTER: Noberini R 

PROVIDER: S-EPMC5504751 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature