Dengue Virus NS1 Protein as a Diagnostic Marker: Commercially Available ELISA and Comparison to qRT-PCR and Serological Diagnostic Assays Currently Used by the State of Florida.

ABSTRACT:

Background

The proper management of patients infected with dengue virus requires early detection. Here, real-time molecular assays have proven useful but have limitations, whereas ELISAs that detect antibodies are still favored but results are obtained too late to be of clinical value. The production of DENV NS1 peaks early during infection and its detection can combine the advantages of both diagnostic approaches.

Methods

This study compared assays currently used for detecting DENV infection at the Florida Department of Health including anti-DENV IgM and IgG ELISAs as well as qRT-PCR, against a commercially available DENV NS1 ELISA. These comparisons were made among a group of 21 human sera.

Results

Nine of 14 (64.3%) DENV qRT-PCR+ samples were also DENV NS1+. Intere

SUBMITTER: Ambrose JH 

PROVIDER: S-EPMC5504952 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature