Sandwich probes: two simultaneous reactions for templated nucleic acid detection.

ABSTRACT: Fluorescence-quenched nucleic acid probes with reactive moieties at both the 5' and 3' ends are synthesized and tested for reaction with two adjacent nucleophile-containing DNAs. These probes improve signal to background over singly reactive probes and can discriminate single nucleotide polymorphisms in the target DNA or RNA.

SUBMITTER: Kleinbaum DJ 

PROVIDER: S-EPMC5504956 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature