Elevation of brain-enriched miRNAs in cerebrospinal fluid of patients with acute ischemic stroke.

ABSTRACT:

Background

The purpose of this study was to investigate the potential of cerebrospinal fluid miRNAs as diagnostic biomarkers of acute ischemic stroke using three different profiling techniques in order to identify and bypass any influence from technical variation.

Methods

Cerebrospinal fluid (CSF) from patients with acute ischemic stroke (n = 21) and controls (n = 21) was collected by lumbar puncture. miRNA analysis was performed with three different methods: 1) Trizol RNA extraction followed by Illumina Next Generation Sequencing (NGS) on all small RNAs, 2) Exiqon RNA extraction protocol and miRNA qPCR assays, and 3) validation of 24 selected miRNAs with Norgen Biotek RNA extraction protocol and Applied Biosystems qPCR assays.

Results

NGS detected 71 fr

SUBMITTER: Sorensen SS 

PROVIDER: S-EPMC5504978 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature