Inferring clonal structure in HTLV-1-infected individuals: towards bridging the gap between analysis and visualization.

ABSTRACT:

Background

Human T cell leukemia virus type 1 (HTLV-1) causes adult T cell leukemia (ATL) in a proportion of infected individuals after a long latency period. Development of ATL is a multistep clonal process that can be investigated by monitoring the clonal expansion of HTLV-1-infected cells by isolation of provirus integration sites. The clonal composition (size, number, and combinations of clones) during the latency period in a given infected individual has not been clearly elucidated.

Methods

We used high-throughput sequencing technology coupled with a tag system for isolating integration sites and measuring clone sizes from 60 clinical samples. We assessed the role of clonality and clone size dynamics in ATL onset by modeling data from high-throughput monitoring of HTLV-

SUBMITTER: Farmanbar A 

PROVIDER: S-EPMC5505134 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature