Human immune compartment comparisons: Optimization of proliferative assays for blood and gut T lymphocytes.

ABSTRACT: The accumulation of peripheral blood late-differentiated memory CD8 T cells with features of replicative (cellular) senescence, including inability to proliferate in vitro, has been extensively studied. Importantly, the abundance of these cells is directly correlated with increased morbidity and mortality in older persons. Of note, peripheral blood contains only 2% of the total body lymphocyte population. By contrast, the gut-associated lymphoid tissue (GALT) is the most extensive lymphoid organ, housing up to 60% of total body lymphocytes, but has never been assessed with respect to senescence profiles. We report here the development of a method for measuring and comparing proliferative capacity of peripheral blood and gut colorectal mucosa-derived CD8 T cells. The protocol involves a 5-d

SUBMITTER: Dock J 

PROVIDER: S-EPMC5505254 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature