Novel assay reveals a large, inducible, replication-competent HIV-1 reservoir in resting CD4⁺ T cells.

ABSTRACT: Although antiretroviral therapy can suppress HIV-1 infection to undetectable levels of plasma viremia, integrated latent HIV-1 genomes that encode replication-competent virus persist in resting CD4⁺ T cells. This latent HIV-1 reservoir represents a major barrier to a cure. Currently, there are substantial efforts to identify therapeutic approaches that will eliminate or reduce the size of this latent HIV-1 reservoir. In this regard, a sensitive assay that can accurately and rapidly quantify inducible, replication-competent latent HIV-1 from resting CD4⁺ T cells is essential for HIV-1 eradication studies. Here we describe a reporter cell-based assay to quantify inducible, replication-competent latent HIV-1. This assay has several advantages over existing technology in

SUBMITTER: Sanyal A 

PROVIDER: S-EPMC5505781 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature