An investigative graduate laboratory course for teaching modern DNA techniques.

ABSTRACT: This graduate-level DNA methods laboratory course is designed to model a discovery-based research project and engages students in both traditional DNA analysis methods and modern recombinant DNA cloning techniques. In the first part of the course, students clone the Drosophila ortholog of a human disease gene of their choosing using Gateway^® cloning. In the second part of the course, students examine the expression of their gene of interest in human cell lines by reverse transcription PCR and learn how to analyze data from quantitative reverse transcription PCR (qRT-PCR) experiments. The adaptability of the Gateway^® cloning system is ideally suited for students to design and create different types of expression constructs to achieve a particular experimental goal (e.g

SUBMITTER: de Lencastre A 

PROVIDER: S-EPMC5505783 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature