Exosomal MicroRNA Transfer Into Macrophages Mediates Cellular Postconditioning.

ABSTRACT:

Background

Cardiosphere-derived cells (CDCs) confer cardioprotection in acute myocardial infarction by distinctive macrophage (Mϕ) polarization. Here we demonstrate that CDC-secreted exosomes (CDC_exo) recapitulate the cardioprotective effects of CDC therapy known as cellular postconditioning.

Methods

Rats and pigs underwent myocardial infarction induced by ischemia/reperfusion before intracoronary infusion of CDC_exo, inert fibroblast exosomes (Fb_exo; control), or vehicle. Two days later, infarct size was quantified. Macrophages were isolated from cardiac tissue or bone marrow for downstream analyses. RNA sequencing was used to determine exosome content and alterations in gene expression profiles in Mϕ.

Results

Administration of CDC

SUBMITTER: de Couto G 

PROVIDER: S-EPMC5505791 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature