Transglycosylation by a chitinase from Enterobacter cloacae subsp. cloacae generates longer chitin oligosaccharides.

ABSTRACT: Humans have exploited natural resources for a variety of applications. Chitin and its derivative chitin oligosaccharides (CHOS) have potential biomedical and agricultural applications. Availability of CHOS with the desired length has been a major limitation in the optimum use of such natural resources. Here, we report a single domain hyper-transglycosylating chitinase, which generates longer CHOS, from Enterobacter cloacae subsp. cloacae 13047 (EcChi1). EcChi1 was optimally active at pH 5.0 and 40 °C with a K_m of 15.2 mg ml^-1, and k _cat/K_m of 0.011× 10² mg^-1 ml min^-1 on colloidal chitin. The profile of the hydrolytic products, major product being chitobiose, released from CHOS indicated that EcChi1 was an endo-act

SUBMITTER: Mallakuntla MK 

PROVIDER: S-EPMC5505975 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature