Dual non-contiguous peptide occupancy of HLA class I evoke antiviral human CD8 T cell response and form neo-epitopes with self-antigens.

ABSTRACT: Host CD8 T cell response to viral infections involves recognition of 8-10-mer peptides presented by MHC-I molecules. However, proteasomes generate predominantly 2-7-mer peptides, but the role of these peptides is largely unknown. Here, we show that single short peptides of <8-mer from Latent Membrane Protein 2 (LMP2) of Epstein Barr Virus (EBV) can bind HLA-A*11:01 and stimulate CD8⁺ cells. Surprisingly, two peptide fragments between 4-7-mer derived from LMP2_(340-349) were able to complement each other, forming combination epitopes that can stimulate specific CD8⁺ T cell responses. Moreover, peptides from self-antigens can complement non-self peptides within the HLA binding cleft, forming neoepitopes. Solved structures of a tetra-complex comprising two pept

SUBMITTER: Xiao Z 

PROVIDER: S-EPMC5505988 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature