All muscarinic acetylcholine receptors (M₁-M₅) are expressed in murine brain microvascular endothelium.

ABSTRACT: Clinical and experimental studies indicate that muscarinic acetylcholine receptors are potential pharmacological targets for the treatment of neurological diseases. Although these receptors have been described in human, bovine and rat cerebral microvascular tissue, a subtype functional characterization in mouse brain endothelium is lacking. Here, we show that all muscarinic acetylcholine receptors (M₁-M₅) are expressed in mouse brain microvascular endothelial cells. The mRNA expression of M₂, M₃, and M₅ correlates with their respective protein abundance, but a mismatch exists for M₁ and M₄ mRNA versus protein levels. Acetylcholine activates calcium transients in brain endothelium via muscarinic, but not nicotinic,

SUBMITTER: Radu BM 

PROVIDER: S-EPMC5506046 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature