Barcode ITS2: a useful tool for identifying Trachelospermum jasminoides and a good monitor for medicine market.

ABSTRACT: Trachelospermum jasminoides is commonly used in traditional Chinese medicine. However, the use of the plant's local alternatives is frequent, causing potential clinical problems. The T. jasminoides sold in the medicine market is commonly dried and sliced, making traditional identification methods difficult. In this study, the ITS2 region was evaluated on 127 sequences representing T. jasminoides and its local alternatives according to PCR and sequencing rates, intra- and inter-specific divergences, secondary structure, and discrimination capacity. Results indicated the 100% success rates of PCR and sequencing and the obvious presence of a barcoding gap. Results of BLAST 1, nearest distance and neighbor-joining tree methods showed that barcode ITS2 could successfully identify all the texted

SUBMITTER: Yu N 

PROVIDER: S-EPMC5506054 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature