Multimodal imaging quality control of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells.

ABSTRACT: Current imaging techniques for the characterization of differentiated corneal limbal stem cells are destructive and cannot be used in eye bank for monitoring the regenerated epithelium in culture. We presented a minimally invasive, multimodal, marker-free imaging method for the investigation of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells. Two-photon fluorescence and harmonic generation signals were collected from specimens in culture and used for evaluating the structure and morphology of epithelia cultured on two different bio-scaffolds; in addition, donor human corneal tissues were used as controls. The method provided reliable information on the organization of cellular and extracellular components of biomaterial substrates and was highly sensiti

SUBMITTER: Lombardo M 

PROVIDER: S-EPMC5506064 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature