Interferon-Gamma DNA Methylation Is Affected by Mycophenolic Acid but Not by Tacrolimus after T-Cell Activation.

ABSTRACT: Immunosuppressive drug therapy is required to treat patients with autoimmune disease and patients who have undergone organ transplantation. The main targets of the immunosuppressive drugs tacrolimus and mycophenolic acid (MPA; the active metabolite of mycophenolate mofetil) are T cells. It is currently unknown whether these immunosuppressive drugs have an effect on DNA methylation-an epigenetic regulator of cellular function. Here, we determined the effect of tacrolimus and MPA on DNA methylation of the gene promoter region of interferon gamma (IFNγ), a pro-inflammatory cytokine. Total T cells, naive T cells (CCR7⁺CD45RO^-), and memory T cells (CD45RO⁺ and CCR7^-CD45RO^-) were isolated from CMV seropositive healthy controls and stimulated

SUBMITTER: Peters FS 

PROVIDER: S-EPMC5506181 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature