Buwchitin: A Ruminal Peptide with Antimicrobial Potential against Enterococcus faecalis.

ABSTRACT: Antimicrobial peptides (AMPs) are gaining popularity as alternatives for treatment of bacterial infections and recent advances in omics technologies provide new platforms for AMP discovery. We sought to determine the antibacterial activity of a novel antimicrobial peptide, buwchitin, against Enterococcus faecalis. Buwchitin was identified from a rumen bacterial metagenome library, cloned, expressed and purified. The antimicrobial activity of the recombinant peptide was assessed using a broth microdilution susceptibility assay to determine the peptide's killing kinetics against selected bacterial strains. The killing mechanism of buwchitin was investigated further by monitoring its ability to cause membrane depolarization (diSC₃(5) method) and morphological changes

SUBMITTER: Oyama LB 

PROVIDER: S-EPMC5506224 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature