Transgenic cotton expressing Cry10Aa toxin confers high resistance to the cotton boll weevil.

ABSTRACT: Genetically modified (GM) cotton plants that effectively control cotton boll weevil (CBW), which is the most destructive cotton insect pest in South America, are reported here for the first time. This work presents the successful development of a new GM cotton with high resistance to CBW conferred by Cry10Aa toxin, a protein encoded by entomopathogenic Bacillus thuringiensis (Bt) gene. The plant transformation vector harbouring cry10Aa gene driven by the cotton ubiquitination-related promoter uceA1.7 was introduced into a Brazilian cotton cultivar by biolistic transformation. Quantitative PCR (qPCR) assays revealed high transcription levels of cry10Aa in both T₀ GM cotton leaf and flower bud tissues. Southern blot and qPCR-based 2^-ΔΔCt analyses revealed that T_0</

SUBMITTER: Ribeiro TP 

PROVIDER: S-EPMC5506659 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature