The expression level of C19MC miRNAs in early pregnancy and in response to viral infection.

ABSTRACT:

Introduction

We have previously shown that miRNAs produced from the Chromosome 19 MiRNA Cluster (C19MC), which are expressed almost exclusively in primate trophoblasts and are released into the maternal circulation, reduce viral replication in non-placental cells and can modulate migratory behavior of extravillous trophoblast. We sought to define the expression pattern of C19MC miRNA in early pregnancy and in response to viral infection in vitro and in vivo.

Methods

We prospectively followed women undergoing in vitro fertilization (IVF) and determined their blood level of C19MC miRNA using RT-qPCR. To examine the effect of viral exposure on C19MC miRNAs expression, we used three systems: (1) a transgenic mouse overexpressing the C19MC cluster and exposed to Togaviridae durin

SUBMITTER: Dumont TMF 

PROVIDER: S-EPMC5506831 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature