Hypermethylated DNA, a circulating biomarker for colorectal cancer detection.

ABSTRACT:

Background

Colorectal cancer (CRC) is one of the most common cancers in the western world. Screening is an efficient method of reducing cancer-related mortality. Molecular biomarkers for cancer in general and CRC in particular have been proposed, and hypermethylated DNA from stool or blood samples are already implemented as biomarkers for CRC screening. We aimed to evaluate the performance of proven hypermethylated DNA promoter regions as plasma based biomarkers for CRC detection.

Methods

We conducted a cross-sectional case-control study of 193 CRC patients and 102 colonoscopy-verified healthy controls. Using methylation specific polymerase chain reaction, we evaluated 30 DNA promoter regions previously found to be CRC specific. We used multivariable logistic regression with

SUBMITTER: Rasmussen SL 

PROVIDER: S-EPMC5507256 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature