In vivo calcium imaging from dentate granule cells with wide-field fluorescence microscopy.

ABSTRACT: A combination of genetically-encoded calcium indicators and micro-optics has enabled monitoring of large-scale dynamics of neuronal activity from behaving animals. In these studies, wide-field microscopy is often used to visualize neural activity. However, this method lacks optical sectioning capability, and therefore its axial resolution is generally poor. At present, it is unclear whether wide-field microscopy can visualize activity of densely packed small neurons at cellular resolution. To examine the applicability of wide-field microscopy for small-sized neurons, we recorded calcium activity of dentate granule cells having a small soma diameter of approximately 10 micrometers. Using a combination of high numerical aperture (0.8) objective lens and independent component analysis-based i

SUBMITTER: Hayashi Y 

PROVIDER: S-EPMC5507494 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature