Dynamic microtubule association of Doublecortin X (DCX) is regulated by its C-terminus.

ABSTRACT: Doublecortin X (DCX), known to be essential for neuronal migration and cortical layering in the developing brain, is a 40 kDa microtubule (MT)-associated protein. DCX directly interacts with MTs via its two structured doublecortin (DC) domains, but the dynamics of this association and the possible regulatory roles played by the flanking unstructured regions remain poorly defined. Here, we employ quantitative fluorescence recovery after photobleaching (FRAP) protocols in living cells to reveal that DCX shows remarkably rapid and complete exchange within the MT network but that the removal of the C-terminal region significantly slows this exchange. We further probed how MT organization or external stimuli could additionally modulate DCX exchange dynamics. MT depolymerisation (nocodazole trea

SUBMITTER: Moslehi M 

PROVIDER: S-EPMC5507856 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature