Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side.

ABSTRACT: Communication between embryo and maternal endometrium occurs during a specific time frame in which implantation is possible. Here we demonstrate for the first time that conditioned media from non-manipulated human embryos cultured in vitro for 3 days or up to the blastocyst stage contain extracellular vesicles (EVs) with a diameter of 50 to 200 nm and bearing the traditional microvesicle and exosome marker proteins CD63, CD9 and ALIX. The embryonic origin of these EVs has been confirmed by the presence of stemness gene transcripts and their enrichment in the non-classical HLA-G protein. NANOG and POU5F1 transcripts were shown to be contained in vesicles deriving from embryos at different stages of development. In line with a higher detection rate of the HLA-G protein in blastocysts compare

SUBMITTER: Giacomini E 

PROVIDER: S-EPMC5507879 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature