Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation.

ABSTRACT: Clostridium perfringens α-toxin induces hemolysis of erythrocytes from various species, but it has not been elucidated whether the toxin affects erythropoiesis. In this study, we treated bone marrow cells (BMCs) from mice with purified α-toxin and found that TER119⁺ erythroblasts were greatly decreased by the treatment. A variant α-toxin defective in enzymatic activities, phospholipase C and sphingomyelinase, had no effect on the population of erythroblasts, demonstrating that the decrease in erythroblasts was dependent of its enzymatic activities. α-Toxin reduced the CD71⁺TER119⁺ and CD71^-TER119⁺ cell populations but not the CD71⁺TER119^- cell population. In addition, α-toxin decreased the number of colony-forming

SUBMITTER: Takagishi T 

PROVIDER: S-EPMC5507896 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature