Massively parallel whole genome amplification for single-cell sequencing using droplet microfluidics.

ABSTRACT: Massively parallel single-cell genome sequencing is required to further understand genetic diversities in complex biological systems. Whole genome amplification (WGA) is the first step for single-cell sequencing, but its throughput and accuracy are insufficient in conventional reaction platforms. Here, we introduce single droplet multiple displacement amplification (sd-MDA), a method that enables massively parallel amplification of single cell genomes while maintaining sequence accuracy and specificity. Tens of thousands of single cells are compartmentalized in millions of picoliter droplets and then subjected to lysis and WGA by passive droplet fusion in microfluidic channels. Because single cells are isolated in compartments, their genomes are amplified to saturation without contaminatio

SUBMITTER: Hosokawa M 

PROVIDER: S-EPMC5507899 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature