Actomyosin and vimentin cytoskeletal networks regulate nuclear shape, mechanics and chromatin organization.

ABSTRACT: The regulation of nuclear state by the cytoskeleton is an important part of cellular function. Actomyosin stress fibres, microtubules and intermediate filaments have distinct and complementary roles in integrating the nucleus into its environment and influencing its mechanical state. However, the interconnectedness of cytoskeletal networks makes it difficult to dissect their individual effects on the nucleus. We use simple image analysis approaches to characterize nuclear state, estimating nuclear volume, Poisson's ratio, apparent elastic modulus and chromatin condensation. By combining them with cytoskeletal quantification, we assess how cytoskeletal organization regulates nuclear state. We report for a number of cell types that nuclei display auxetic properties. Furthermore, stress fibre

SUBMITTER: Keeling MC 

PROVIDER: S-EPMC5507932 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature