Deconvolution of the Response to Bacillus Calmette-Guerin Reveals NF-κB-Induced Cytokines As Autocrine Mediators of Innate Immunity.

ABSTRACT: Bacillus Calmette-Guérin (BCG) is used as a vaccine and diagnostic test for tuberculosis, as well as immunotherapy in the treatment of bladder cancer. While clinically useful, the response to mycobacterial stimulation is complex and the induced protein signature remains poorly defined. We characterized the cell types directly engaged by BCG, as well as the induced cytokine loops that transmit signal(s) to bystander cells. Standardized whole-blood stimulations and mechanistic studies on single and purified cell populations identified distinct patterns of activation in monocytes as compared to neutrophils and invariant lymphocyte populations. Deconvoluting the role of Toll-like receptor 2/4 and Dectin-1/2 in the inflammatory response to BCG, we revealed Dectin-1/2 as dominant in neutrophils

SUBMITTER: Bisiaux A 

PROVIDER: S-EPMC5507989 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature