Visualization of tumor heterogeneity by in situ padlock probe technology in colorectal cancer.

ABSTRACT: Tumor heterogeneity is considered a major cause for therapy resistance in colorectal cancer. Sub-populations of cells with different genetic alterations may exist in spatially distinct areas. Upon therapy, resistant sub-clones may enrich and ultimately lead to disease progression. Although ample data are available on tumors which are heterogeneous on a morphological level, only little is known about morphologically homogeneous tumors. We aimed to investigate if morphologically homogeneous colorectal cancer can harbor a heterogeneous genetic landscape. We chose to microdissect six morphologically homogeneous colorectal carcinomas into several areas and performed next-generation sequencing (NGS) to identify tumors with genetic heterogeneity. We then applied an mRNA-based in situ mutation det

SUBMITTER: El-Heliebi A 

PROVIDER: S-EPMC5508037 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature