Organometallic activation of a fluorogen for templated nucleic acid detection.

ABSTRACT: A nucleic acid detection scheme that employs DNA-mediated delivery of an organomercury activator to unmask a fluorophore is described. The approach relies on adjacent hybridization of two oligonucleotide conjugates containing organomercury and caged rhodamine functionalities. Postsynthetic conjugation of amino-modified DNAs enabled efficient preparation of these probes. Complementary DNA templates yielded fluorescence signals arising from metal-assisted rhodamine uncaging.

SUBMITTER: Franzini RM 

PROVIDER: S-EPMC5508869 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature