On-chip human microvasculature assay for visualization and quantification of tumor cell extravasation dynamics.

ABSTRACT: Distant metastasis, which results in >90% of cancer-related deaths, is enabled by hematogenous dissemination of tumor cells via the circulation. This requires the completion of a sequence of complex steps including transit, initial arrest, extravasation, survival and proliferation. Increased understanding of the cellular and molecular players enabling each of these steps is key to uncovering new opportunities for therapeutic intervention during early metastatic dissemination. As a protocol extension, this article describes an adaptation to our existing protocol describing a microfluidic platform that offers additional applications. This protocol describes an in vitro model of the human microcirculation with the potential to recapitulate discrete steps of early metastatic seeding, including

SUBMITTER: Chen MB 

PROVIDER: S-EPMC5509465 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature