Acoustic-transfection for genomic manipulation of single-cells using high frequency ultrasound.

ABSTRACT: Efficient intracellular delivery of biologically active macromolecules has been a challenging but important process for manipulating live cells for research and therapeutic purposes. There have been limited transfection techniques that can deliver multiple types of active molecules simultaneously into single-cells as well as different types of molecules into physically connected individual neighboring cells separately with high precision and low cytotoxicity. Here, a high frequency ultrasound-based remote intracellular delivery technique capable of delivery of multiple DNA plasmids, messenger RNAs, and recombinant proteins is developed to allow high spatiotemporal visualization and analysis of gene and protein expressions as well as single-cell gene editing using clustered regularly inters

SUBMITTER: Yoon S 

PROVIDER: S-EPMC5509725 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature