High-Throughput Microplate-Based Assay to Monitor Plasma Membrane Wounding and Repair.

ABSTRACT: The plasma membrane of mammalian cells is susceptible to disruption by mechanical and biochemical damages that frequently occur within tissues. Therefore, efficient and rapid repair of the plasma membrane is essential for maintaining cellular homeostasis and survival. Excessive damage of the plasma membrane and defects in its repair are associated with pathological conditions such as infections, muscular dystrophy, heart failure, diabetes, and lung and neurodegenerative diseases. The molecular events that remodel the plasma membrane during its repair remain poorly understood. In the present work, we report the development of a quantitative high-throughput assay that monitors the efficiency of the plasma membrane repair in real time using a sensitive microplate reader. In this assay, the pl

SUBMITTER: Pathak-Sharma S 

PROVIDER: S-EPMC5509797 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature