A new assay based on terminal restriction fragment length polymorphism of homocitrate synthase gene fragments for Candida species identification.

ABSTRACT: Candida sp. have been responsible for an increasing number of infections, especially in patients with immunodeficiency. Species-specific differentiation of Candida sp. is difficult in routine diagnosis. This identification can have a highly significant association in therapy and prophylaxis. This work has shown a new application of the terminal restriction fragment length polymorphism (t-RFLP) method in the molecular identification of six species of Candida, which are the most common causes of fungal infections. Specific for fungi homocitrate synthase gene was chosen as a molecular target for amplification. The use of three restriction enzymes, DraI, RsaI, and BglII, for amplicon digestion can generate species-specific fluorescence labeled DNA fragment profiles, which can be used to determ

SUBMITTER: Szemiako K 

PROVIDER: S-EPMC5509809 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature