Quantitative High-Throughput Screening Using a Coincidence Reporter Biocircuit.

ABSTRACT: Reporter-biased artifacts-i.e., compounds that interact directly with the reporter enzyme used in a high-throughput screening (HTS) assay and not the biological process or pharmacology being interrogated-are now widely recognized to reduce the efficiency and quality of HTS used for chemical probe and therapeutic development. Furthermore, narrow or single-concentration HTS perpetuates false negatives during primary screening campaigns. Titration-based HTS, or quantitative HTS (qHTS), and coincidence reporter technology can be employed to reduce false negatives and false positives, respectively, thereby increasing the quality and efficiency of primary screening efforts, where the number of compounds investigated can range from tens of thousands to millions. The three protocols described here

SUBMITTER: Schuck BW 

PROVIDER: S-EPMC5510169 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature