Ontology highlight
ABSTRACT:
SUBMITTER: Jo J
PROVIDER: S-EPMC5510242 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Cell stem cell 20160728 2
Recent advances in 3D culture systems have led to the generation of brain organoids that resemble different human brain regions; however, a 3D organoid model of the midbrain containing functional midbrain dopaminergic (mDA) neurons has not been reported. We developed a method to differentiate human pluripotent stem cells into a large multicellular organoid-like structure that contains distinct layers of neuronal cells expressing characteristic markers of human midbrain. Importantly, we detected ...[more]