Exogenous Fatty Acids Are the Preferred Source of Membrane Lipids in Proliferating Fibroblasts.

ABSTRACT: Cellular proliferation requires the formation of new membranes. It is often assumed that the lipids needed for these membranes are synthesized mostly de novo. Here, we show that proliferating fibroblasts prefer to take up palmitate from the extracellular environment over synthesizing it de novo. Relative to quiescent fibroblasts, proliferating fibroblasts increase their uptake of palmitate, decrease fatty acid degradation, and instead direct more palmitate to membrane lipids. When exogenous palmitate is provided in the culture media at physiological concentrations, de novo synthesis accounts for only a minor fraction of intracellular palmitate in proliferating fibroblasts as well as proliferating HeLa and H460 cells. Blocking fatty acid uptake decreased the proliferation rate of fibroblast

SUBMITTER: Yao CH 

PROVIDER: S-EPMC5510604 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature