Quantitative Brightness Analysis of Protein Oligomerization in the Nuclear Envelope.

ABSTRACT: Brightness analysis of fluorescence fluctuation experiments has been used to successfully measure the oligomeric state of proteins at the plasma membrane, in the nucleoplasm, and in the cytoplasm of living cells. Here we extend brightness analysis to the nuclear envelope (NE), a double membrane barrier separating the cytoplasm from the nucleoplasm. Results obtained by applying conventional brightness analysis to fluorescently tagged proteins within the NE exhibited an unusual concentration dependence. Similarly, the autocorrelation function of the fluorescence fluctuations exhibited unexpected changes with protein concentration. These observations motivated the application of mean-segmented Q analysis, which identified the existence of a fluctuation process distinct from molecular diffusio

SUBMITTER: Hennen J 

PROVIDER: S-EPMC5510813 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature