Suppression of cell migration by phospholipase C-related catalytically inactive protein-dependent modulation of PI3K signalling.

ABSTRACT: The metabolic processes of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P₂] into PI(3,4,5)P₃ and the subsequent PI(3,4,5)P₃ signalling are involved in cell migration. Dysfunctions in the control of this pathway can cause human cancer cell migration and metastatic growth. Here we investigated whether phospholipase C-related catalytically inactive protein (PRIP), a PI(4,5)P₂-binding protein, regulates cancer cell migration. PRIP overexpression in MCF-7 and BT-549 human breast cancer cells inhibited cell migration in vitro and metastasis development in vivo. Overexpression of the PRIP pleckstrin homology domain, a PI(4,5)P₂ binding motif, in MCF-7 cells caused significant suppression of cell migration. Consistent with these results, in c

SUBMITTER: Asano S 

PROVIDER: S-EPMC5511194 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature