Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping.

ABSTRACT: Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci.

SUBMITTER: Morgan SL 

PROVIDER: S-EPMC5511349 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature