Stone former urine proteome demonstrates a cationic shift in protein distribution compared to normal.

ABSTRACT: Many urine proteins are found in calcium oxalate stones, yet decades of research have failed to define the role of urine proteins in stone formation. This urine proteomic study compares the relative amounts of abundant urine proteins between idiopathic calcium oxalate stone forming and non-stone forming (normal) cohorts to identify differences that might correlate with disease. Random mid-morning urine samples were collected following informed consent from 25 stone formers and 14 normal individuals. Proteins were isolated from urine using ultrafiltration. Urine proteomes for each sample were characterized using label-free spectral counting mass spectrometry, so that urine protein relative abundances could be compared between the two populations. A total of 407 unique proteins were identifi

SUBMITTER: Kolbach-Mandel AM 

PROVIDER: S-EPMC5511579 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature