Gene Expression and Molecular Characterization of a Xylanase from Chicken Cecum Metagenome.

ABSTRACT: A xylanase gene xynA_MG1 with a 1,116-bp open reading frame, encoding an endo-β-1,4-xylanase, was cloned from a chicken cecum metagenome. The translated XynA_MG1 protein consisted of 372 amino acids including a putative signal peptide of 23 amino acids. The calculated molecular mass of the mature XynA_MG1 was 40,013 Da, with a theoretical pI value of 5.76. The amino acid sequence of XynA_MG1 showed 59% identity to endo-β-1,4-xylanase from Prevotella bryantii and Prevotella ruminicola and 58% identity to that from Prevotella copri. XynA_MG1 has two conserved motifs, DVVNE and TEXD, containing two active site glutamates and an invariant asparagine, characteristic of GH10 family xylanase. The

SUBMITTER: Al-Darkazali H 

PROVIDER: S-EPMC5511640 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature