Antitumor Effect of KX-01 through Inhibiting Src Family Kinases and Mitosis.

ABSTRACT:

Purpose

KX-01 is a novel dual inhibitor of Src and tubulin. Unlike previous Src inhibitors that failed to show clinical benefit during treatment of breast cancer, KX-01 can potentially overcome the therapeutic limitations of current Src inhibitors through inhibition of both Src and tubulin. The present study further evaluates the activity and mechanism of KX-01 in vitro and in vivo.

Materials and methods

The antitumor effect of KX-01 in triple negative breast cancer (TNBC) cell lines was determined by MTT assay. Wound healing and immunofluorescence assays were performed to evaluate the action mechanisms of KX-01. Changes in the cell cycle and molecular changes induced by KX-01 were also evaluated. A MDA-MB-231 mouse xenograft model was used to demonstrate the <

SUBMITTER: Kim S 

PROVIDER: S-EPMC5512373 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature