Proton Pump Inhibition Enhances the Cytotoxicity of Paclitaxel in Cervical Cancer.

ABSTRACT:

Purpose

This study was conducted to investigate whether a proton pump inhibitor (PPI) could enhance chemosensitivity via the inhibition of vacuolar-type H⁺ ATPase (V-ATPase) in cervical cancer.

Materials and methods

The expression of V-ATPase was evaluated in 351 formalin-fixed, paraffin-embedded human cervical cancer tissues using immunohistochemistry and compared with clinicopathologic risk factors for disease prognosis. The influence of cell proliferation and apoptosis following V-ATPase siRNA transfection or esomeprazole pretreatment was assessed in cervical cancer cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and enzyme-linked immunosorbent assay, respectively.

Results

Immunohistochemical analysis revealed that V-ATPase

SUBMITTER: Song T 

PROVIDER: S-EPMC5512380 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature