Genetically encoded calcium indicators for studying long-term calcium dynamics during apoptosis.

ABSTRACT: Intracellular calcium release is essential for regulating almost all cellular functions. Specific spatio-temporal patterns of cytosolic calcium elevations are critical determinants of cell fate in response to pro-apoptotic cellular stressors. As the apoptotic program can take hours or days, measurement of long-term calcium dynamics are essential for understanding the mechanistic role of calcium in apoptotic cell death. Due to the technical limitations of using calcium-sensitive dyes to measure cytosolic calcium little is known about long-term calcium dynamics in living cells after treatment with apoptosis-inducing drugs. Genetically encoded calcium indicators could potentially overcome some of the limitations of calcium-sensitive dyes. Here, we compared the performance of the genetically e

SUBMITTER: Garcia MI 

PROVIDER: S-EPMC5512452 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature