Utility of quantitative micro-computed tomographic analysis in zebrafish to define gene function during skeletogenesis.

ABSTRACT: The zebrafish is a powerful experimental model to investigate the genetic and morphologic basis of vertebrate development. Analysis of skeletogenesis in this fish is challenging as a result of the small size of the developing and adult zebrafish. Many of the bones of small fishes such as the zebrafish and medaka are quite thin, precluding many standard assays of bone quality and morphometrics commonly used on bones of larger animals. Microcomputed tomography (microCT) is a common imaging technique used for detailed analysis of the skeleton of the zebrafish and determination of mutant phenotypes. However, the utility of this modality for analysis of the zebrafish skeleton, and the effect of inherent variation among individual zebrafish, including variables such as sex, age and strain, is no

SUBMITTER: Charles JF 

PROVIDER: S-EPMC5512604 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature