Clonal competition in BcrAbl-driven leukemia: how transplantations can accelerate clonal conversion.

ABSTRACT:

Background

Clonal competition in cancer describes the process in which the progeny of a cell clone supersedes or succumbs to other competing clones due to differences in their functional characteristics, mostly based on subsequently acquired mutations. Even though the patterns of those mutations are well explored in many tumors, the dynamical process of clonal selection is underexposed.

Methods

We studied the dynamics of clonal competition in a BcrAbl-induced leukemia using a γ-retroviral vector library encoding the oncogene in conjunction with genetic barcodes. To this end, we studied the growth dynamics of transduced cells on the clonal level both in vitro and in vivo in transplanted mice.

Results

While we detected moderate changes in clonal abundancies in vitro, we

SUBMITTER: Cornils K 

PROVIDER: S-EPMC5512731 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature