Retinal Pigment Epithelial Cells are a Potential Reservoir for Ebola Virus in the Human Eye.

ABSTRACT:

Purpose

Success of Ebola virus (EBOV) as a human pathogen relates at the molecular level primarily to blockade the host cell type I interferon (IFN) antiviral response. Most individuals who survive Ebola virus disease (EVD) develop a chronic disease syndrome: approximately one-quarter of survivors suffer from uveitis, which has been associated with presence of EBOV within the eye. Clinical observations of post-Ebola uveitis indicate involvement of retinal pigment epithelial cells.

Methods

We inoculated ARPE-19 human retinal pigment epithelial cells with EBOV, and followed course of infection by immunocytochemistry and measurement of titer in culture supernatant. To interrogate transcriptional responses of infected cells, we combined RNA sequencing with in silico pathway, gen

SUBMITTER: Smith JR 

PROVIDER: S-EPMC5512973 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature