The M-phase specific hyperphosphorylation of Staufen2 involved the cyclin-dependent kinase CDK1.

ABSTRACT:

Background

Staufen2 (STAU2) is an RNA-binding protein involved in the post-transcriptional regulation of gene expression. This protein was shown to be required for organ formation and cell differentiation. Although STAU2 functions have been reported in neuronal cells, its role in dividing cells remains deeply uncharacterized. Especially, its regulation during the cell cycle is completely unknown.

Results

In this study, we showed that STAU2 isoforms display a mitosis-specific slow migration pattern on SDS-gels in all tested transformed and untransformed cell lines. Deeper analyses in hTert-RPE1 and HeLa cells further indicated that the slow migration pattern of STAU2 isoforms is due to phosphorylation. Time course studies showed that STAU2 phosphorylation occurs before promet

SUBMITTER: Beaujois R 

PROVIDER: S-EPMC5513041 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature