Development and validation of novel biomarker assays for osteoarthritis.

ABSTRACT:

Background

Osteoarthritis (OA) is the most common chronic joint disease usually diagnosed at relatively advanced stages when there is irreparable damage to the joint(s). Recently, we have identified two novel biomarkers C3f and V65 which appear to be OA-specific and therefore potential markers of early disease. We report the development of immunoassays for quantitative measure of these two novel biomarkers.

Method

Monoclonal and polyclonal antibodies were generated by immunising mouse and rabbits respectively with peptide-carrier conjugates of C3f and V65. Affinity purified antibodies were used for immunoassays development and assays validated using serum from OA patients and controls.

Results

The ELISAs developed showed spiked recovery of up to 96% for C3f and V65 pe

SUBMITTER: Ourradi K 

PROVIDER: S-EPMC5513499 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature