Profound loss of esophageal tissue differentiation in patients with eosinophilic esophagitis.

ABSTRACT:

Background

A key question in the allergy field is to understand how tissue-specific disease is manifested. Eosinophilic esophagitis (EoE) is an emerging tissue-specific allergic disease with an unclear pathogenesis.

Objective

Herein we tested the hypothesis that a defect in tissue-specific esophageal genes is an integral part of EoE pathogenesis.

Methods

We interrogated the pattern of expression of esophagus-specific signature genes derived from the Human Protein Atlas in the EoE transcriptome and in EPC2 esophageal epithelial cells. Western blotting and immunofluorescence were used for evaluating expression of esophageal proteins in biopsy specimens from control subjects and patients with active EoE. Whole-exome sequencing was performed to identify mutations in esoph

SUBMITTER: Rochman M 

PROVIDER: S-EPMC5513800 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature